Journal: Materials Today Bio

Article Title: Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling

doi: 10.1016/j.mtbio.2026.102908

Figure Lengend Snippet: Mild reduction of the cell surface promotes cell–matrix adhesion and focal adhesion assembly on adipose-like stiffness hydrogels. (A) Schematic representation of cell culture on collagen type 1-conjugated polyacrylamide hydrogels with adipose tissue-like stiffness. This schematic was created using BioRender.com . (B) Representative immunofluorescence staining for F-actin (red) and pFAK (green). (C-H) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters. scale bar = 50 μm, Data are presented as mean ± 95% confidence interval (CI). ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Briefly, cells were seeded onto collagen type 1-conjugated polyacrylamide hydrogels and cultured in DMEM supplemented with 10% v/v FBS (26140-079, Gibco, Waltham, MA, USA).

Techniques: Cell Culture, Immunofluorescence, Staining